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ProductsBack/Phospho-MYPT1 (Thr696) Polyclonal Antibody
Human HeLa cells probed with MYPT1 (Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Human HeLa cells probed with MYPT1 (Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Human HeLa cells probed with MYPT1 (Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Phospho-MYPT1 (Thr696) Polyclonal Antibody

BS-3287R
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot
DownloadDatasheet
Product group Antibodies
TargetPPP1R12A
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MYPT1 (Thr696) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4659
  • Target name
    PPP1R12A
  • Target description
    protein phosphatase 1 regulatory subunit 12A
  • Target synonyms
    GUBS; M130; MBS; myosin binding subunit; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; MYPT1; protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase myosin-binding subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14974
  • Protein Name
    Protein phosphatase 1 regulatory subunit 12A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R12A antigen affinity purified polyclonal antibody (Catalog # PB9737) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R12A at approximately 130KD. The expected band size for PPP1R12A is at 130KD.
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