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Western Blot analysis of 293T cells using MYPT1 Polyclonal Antibody
Western Blot analysis of 293T cells using MYPT1 Polyclonal Antibody
Western Blot analysis of 293T cells using MYPT1 Polyclonal Antibody

PPP1R12A Antibody

CSB-PA003352
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetPPP1R12A
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPP1R12A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4659
  • Target name
    PPP1R12A
  • Target description
    protein phosphatase 1 regulatory subunit 12A
  • Target synonyms
    GUBS, M130, MBS, MYPT1, protein phosphatase 1 regulatory subunit 12A, myosin binding subunit, myosin phosphatase, target subunit 1, myosin phosphatase-targeting subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A, protein phosphatase myosin-binding subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14974
  • Protein Name
    Protein phosphatase 1 regulatory subunit 12A
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R12A antigen affinity purified polyclonal antibody (Catalog # PB9737) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R12A at approximately 130KD. The expected band size for PPP1R12A is at 130KD.
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