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ProductsBack/Na+/K+-ATPase alpha1 (6D134)

Na+/K+-ATPase alpha1 (6D134)

SC-71639
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATP1A1
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Na+/K+-ATPase alpha1 (6D134)
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID476
  • Target name
    ATP1A1
  • Target description
    ATPase Na+/K+ transporting subunit alpha 1
  • Target synonyms
    CMT2DD, HOMGSMR2, sodium/potassium-transporting ATPase subunit alpha-1, ATPase, Na+/K+ transporting, alpha 1 polypeptide, Na(+)/K(+) ATPase alpha-1 subunit, Na+/K+ ATPase 1, Na, K-ATPase, alpha-A catalytic polypeptide, Na,K-ATPase alpha-1 subunit, Na,K-ATPase catalytic subunit alpha-A protein, sodium pump subunit alpha-1, sodium-potassium ATPase catalytic subunit alpha-1, sodium-potassium-ATPase, alpha 1 polypeptide
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A1 antigen affinity purified polyclonal antibody (Catalog # PB9482) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody (Left) at a dilution of 1:2000 or a goat anti-rabbit IgG-HRP Conjugated secondary antibody (Right) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A1 approximately 100 kDa. The expected band size for ATP1A1 is at 74, 110, 113 kDa.
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