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Lane 1: Human HL60 cell lysates; Lane 2: Human HL60 cell lysates probed with NCR1 Polyclonal Antibody, Unconjugated (bs-41214R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HL60 cell lysates; Lane 2: Human HL60 cell lysates probed with NCR1 Polyclonal Antibody, Unconjugated (bs-41214R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HL60 cell lysates; Lane 2: Human HL60 cell lysates probed with NCR1 Polyclonal Antibody, Unconjugated (bs-41214R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

NCR1 Polyclonal Antibody

bs-41214R
Bioss
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetNCR1
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Overview

  • Supplier
    Bioss
  • Product Name
    NCR1 Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9437
  • Target name
    NCR1
  • Target description
    natural cytotoxicity triggering receptor 1
  • Target synonyms
    CD335, LY94, NK-p46, NKP46, natural cytotoxicity triggering receptor 1, NK cell-activating receptor, lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46), natural killer cell p46-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO76036
  • Protein Name
    Natural cytotoxicity triggering receptor 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCR1 using anti-NCR1 antibody (A01516-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCR1 antigen affinity purified polyclonal antibody (Catalog # A01516-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCR1 at approximately 36KD. The expected band size for NCR1 is at 34KD.
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