Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [HL2511] (GTX638872) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [HL2511] (GTX638872) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638872/GTX638872_45180_20230929_WB_TPM_watermark_23100319_518.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [HL2511] (GTX638872) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![NEK2 antibody [HL2511] detects NEK2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human testis. NEK2 stained by NEK2 antibody [HL2511] (GTX638872) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638872/GTX638872_45180_20231020_IHC-P_23102401_890.webp "NEK2 antibody [HL2511] detects NEK2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human testis. NEK2 stained by NEK2 antibody [HL2511] (GTX638872) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [HL2511] (GTX638872) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NEK2 antibody [HL2511]
GTX638872
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNEK2
Overview
- SupplierGeneTex
- Product NameNEK2 antibody [HL2511]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2511
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4751
- Target nameNEK2
- Target descriptionNIMA related kinase 2
- Target synonymsHsPK21, NEK2A, NLK1, PPP1R111, RP67, serine/threonine-protein kinase Nek2, NIMA (never in mitosis gene a)-related kinase 2, nimA-like protein kinase 1, nimA-related protein kinase 2, protein phosphatase 1, regulatory subunit 111
- HostRabbit
- IsotypeIgG
- Protein IDP51955
- Protein NameSerine/threonine-protein kinase Nek2
- Scientific DescriptionThis gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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