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Western Blot All lanes: NELFB antibody at 2microg/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
Western Blot All lanes: NELFB antibody at 2microg/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
Western Blot All lanes: NELFB antibody at 2microg/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa

NELFB Antibody

CSB-PA03765A0RB
Cusabio
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNELFB
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Overview

  • Supplier
    Cusabio
  • Product Name
    NELFB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25920
  • Target name
    NELFB
  • Target description
    negative elongation factor complex member B
  • Target synonyms
    COBRA1; cofactor of BRCA1; negative elongation factor B; negative elongation factor protein B; NELF-B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WX92
  • Protein Name
    Negative elongation factor B
  • Scientific Description
    Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of COBRA1 using anti-COBRA1 antibody (A12025-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: human MDA-MB-231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COBRA1 antigen affinity purified polyclonal antibody (Catalog # A12025-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COBRA1 at approximately 66KD. The expected band size for COBRA1 is at 66KD.
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