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NELFB Polyclonal Antibody

CAC14048
Biomatik
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNELFB
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Overview

  • Supplier
    Biomatik
  • Product Name
    NELFB Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25920
  • Target name
    NELFB
  • Target description
    negative elongation factor complex member B
  • Target synonyms
    COBRA1; cofactor of BRCA1; negative elongation factor B; negative elongation factor protein B; NELF-B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WX92
  • Protein Name
    Negative elongation factor B
  • Scientific Description
    The NELFB Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, ChIP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COBRA1 using anti-COBRA1 antibody (A12025-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: human MDA-MB-231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COBRA1 antigen affinity purified polyclonal antibody (Catalog # A12025-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COBRA1 at approximately 66KD. The expected band size for COBRA1 is at 66KD.
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Western Blot All lanes: NELFB antibody at 2microg/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
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