More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/NELFB / COBRA1 Antibody

NELFB / COBRA1 Antibody

LS-C747233
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNELFB
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NELFB / COBRA1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 65kDa, while the observed MW by Western blot was 66kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 65kDa, while the observed MW by Western blot was 66kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID25920
  • Target name
    NELFB
  • Target description
    negative elongation factor complex member B
  • Target synonyms
    COBRA1, NELF-B, negative elongation factor B, cofactor of BRCA1, negative elongation factor protein B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Bioss
NELFB Recombinant AntibodyBSM-62878R
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetNELFB
  • SizePrice
Product group Antibodies
Biomatik
NELFB Polyclonal AntibodyCAC14048
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
TargetNELFB
  • SizePrice
Figure 1. Western blot analysis of COBRA1 using anti-COBRA1 antibody (A12025-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: human MDA-MB-231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COBRA1 antigen affinity purified polyclonal antibody (Catalog # A12025-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COBRA1 at approximately 66KD. The expected band size for COBRA1 is at 66KD.
Product group Antibodies
Boster Bio
Anti-COBRA1/NELFB Antibody Picoband(r)A12025-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetNELFB
  • SizePrice
Product group Antibodies
RayBiotech
Anti-COBRA1 (Center) Antibody102-22019
ApplicationsWestern Blot
TargetNELFB
  • SizePrice
Product group Antibodies
GeneTex
COBRA1 antibodyGTX102043
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetNELFB
  • SizePrice
Immunohistochemistry analysis in human skin and liver tissues using HPA020259 antibody. Corresponding NELFB RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-NELFB AntibodyHPA020259
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNELFB
  • SizePrice
Western Blot All lanes: NELFB antibody at 2microg/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
Product group Antibodies
Cusabio
NELFB AntibodyCSB-PA03765A0RB
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetNELFB
  • SizePrice