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NELFB Recombinant Antibody

BSM-62878R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNELFB
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Overview

  • Supplier
    Bioss
  • Product Name
    NELFB Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(ICC)(1:50-200), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID25920
  • Target name
    NELFB
  • Target description
    negative elongation factor complex member B
  • Target synonyms
    COBRA1, NELF-B, negative elongation factor B, cofactor of BRCA1, negative elongation factor protein B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WX92
  • Protein Name
    Negative elongation factor B
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot All lanes: NELFB antibody at 2microg/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
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Figure 1. Western blot analysis of COBRA1 using anti-COBRA1 antibody (A12025-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: human MDA-MB-231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COBRA1 antigen affinity purified polyclonal antibody (Catalog # A12025-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COBRA1 at approximately 66KD. The expected band size for COBRA1 is at 66KD.
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