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IHC analysis of E13.5 mouse brain using Nestin antibody (green) and DAPI (blue).
IHC analysis of E13.5 mouse brain using Nestin antibody (green) and DAPI (blue).
IHC analysis of E13.5 mouse brain using Nestin antibody (green) and DAPI (blue).

Nestin antibody

GTX85457
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetNES
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Overview

  • Supplier
    GeneTex
  • Product Name
    Nestin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Recommended starting dilutions: For IHC: Use at 1:3000 - 1:6000. For ICC/IF:Use at 1:3000 - 1:6000. For WB: Use at 1:5000 - 1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10763
  • Target name
    NES
  • Target description
    nestin
  • Target synonyms
    Nbla00170; nestin
  • Host
    Chicken
  • Isotype
    IgY
  • Protein IDP48681
  • Protein Name
    Nestin
  • Scientific Description
    This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Spatiotemporal Regulation of De Novo and Salvage Purine Synthesis during Brain Development.
    Read more

Datasheet

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Figure 1. Western blot analysis of Nestin using anti-Nestin antibody (PB9874). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nestin antigen affinity purified polyclonal antibody (Catalog # PB9874) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nestin at approximately 230 kDa. The expected band size for Nestin is at 177 kDa.
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