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Neurogranin Antibody (YA1383, Rabbit)

HY-P81638
MedChem Express
TargetNRGN
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Neurogranin Antibody (YA1383, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4900
  • Target name
    NRGN
  • Target description
    neurogranin
  • Target synonyms
    RC3, hng, neurogranin, calmodulin-binding protein, neurogranin (protein kinase C substrate, RC3), protein kinase C substrate
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Neurogranin Antibody (YA1383) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1383), targeting Neurogranin, with a predicted molecular weight of 8 kDa (observed band size: 14-16 kDa). Neurogranin Antibody (YA1383) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Neurogranin/NRGN using anti-Neurogranin/NRGN antibody (A05781-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurogranin/NRGN antigen affinity purified polyclonal antibody (Catalog # A05781-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurogranin/NRGN at approximately 11 kDa. The expected band size for Neurogranin/NRGN is at 8 kDa.
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