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NFAT4 / NFATC3 Antibody

LS-C814936
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetNFATC3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NFAT4 / NFATC3 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:300 - 1:10000), IF (1:300 - 1:10000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4775
  • Target name
    NFATC3
  • Target description
    nuclear factor of activated T cells 3
  • Target synonyms
    NF-AT4c, NFAT4, NFATX, n339260, nuclear factor of activated T-cells, cytoplasmic 3, NF-ATc3, T cell transcription factor NFAT4, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFAT4 using anti-NFAT4 antibody (A02727-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT4 antigen affinity purified polyclonal antibody (Catalog # A02727-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT4 at approximately 115KD. The expected band size for NFAT4 is at 115KD.
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