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Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Rat kidney tissue All lanes: NFATC3 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 116, 113, 78, 81 kDa Observed band size: 116 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Rat kidney tissue All lanes: NFATC3 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 116, 113, 78, 81 kDa Observed band size: 116 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Rat kidney tissue All lanes: NFATC3 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 116, 113, 78, 81 kDa Observed band size: 116 kDa

NFATC3 Antibody

CSB-PA615540LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetNFATC3
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Overview

  • Supplier
    Cusabio
  • Product Name
    NFATC3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4775
  • Target name
    NFATC3
  • Target description
    nuclear factor of activated T cells 3
  • Target synonyms
    NF-AT4c, NFAT4, NFATX, n339260, nuclear factor of activated T-cells, cytoplasmic 3, NF-ATc3, T cell transcription factor NFAT4, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12968
  • Protein Name
    Nuclear factor of activated T-cells, cytoplasmic 3
  • Scientific Description
    Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of NFAT4 using anti-NFAT4 antibody (A02727-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT4 antigen affinity purified polyclonal antibody (Catalog # A02727-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT4 at approximately 115KD. The expected band size for NFAT4 is at 115KD.
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