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NFE2 Antibody (YA1209, Rabbit)

HY-P81464
MedChem Express
TargetNFE2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    NFE2 Antibody (YA1209, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4778
  • Target name
    NFE2
  • Target description
    nuclear factor, erythroid 2
  • Target synonyms
    NF-E2, p45, transcription factor NF-E2 45 kDa subunit, leucine zipper protein NF-E2, nuclear factor (erythroid-derived 2), 45kD, nuclear factor (erythroid-derived 2), 45kDa, nuclear factor, erythroid-derived 2 45 kDa subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    NFE2 Antibody (YA1209) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1209), targeting NFE2. NFE2 Antibody (YA1209) can be used for IHC-P experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NFE2 using anti-NFE2 antibody (A03765-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFE2 antigen affinity purified polyclonal antibody (Catalog # A03765-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFE2 at approximately 41 kDa. The expected band size for NFE2 is at 41 kDa.
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Product group Antibodies
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Immunohistochemistry analysis in human bone marrow and liver tissues using HPA001914 antibody. Corresponding NFE2 RNA-seq data are presented for the same tissues.
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NF-E2 antibody [N1C3] (GTX102698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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