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ProductsBack/NFE2 / p45 Antibody

NFE2 / p45 Antibody

LS-C667138
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNFE2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NFE2 / p45 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IF, IHC, IP, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4778
  • Target name
    NFE2
  • Target description
    nuclear factor, erythroid 2
  • Target synonyms
    NF-E2, p45, transcription factor NF-E2 45 kDa subunit, leucine zipper protein NF-E2, nuclear factor (erythroid-derived 2), 45kD, nuclear factor (erythroid-derived 2), 45kDa, nuclear factor, erythroid-derived 2 45 kDa subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFE2 using anti-NFE2 antibody (A03765-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFE2 antigen affinity purified polyclonal antibody (Catalog # A03765-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFE2 at approximately 41 kDa. The expected band size for NFE2 is at 41 kDa.
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NF-E2 antibody [N1C3] (GTX102698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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