More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/p45 NF-E2 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with p45 NF-E2 Polyclonal Antibody, Unconjugated (bs-22023R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with p45 NF-E2 Polyclonal Antibody, Unconjugated (bs-22023R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with p45 NF-E2 Polyclonal Antibody, Unconjugated (bs-22023R) at 1:200 overnight at 4°C, DAB staining.

p45 NF-E2 Polyclonal Antibody

BS-22023R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetNFE2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    p45 NF-E2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4778
  • Target name
    NFE2
  • Target description
    nuclear factor, erythroid 2
  • Target synonyms
    leucine zipper protein NF-E2; NF-E2; nuclear factor (erythroid-derived 2), 45kD; nuclear factor (erythroid-derived 2), 45kDa; nuclear factor, erythroid-derived 2 45 kDa subunit; p45; p45 NF-E2; transcription factor NF-E2 45 kDa subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16621
  • Protein Name
    Transcription factor NF-E2 45 kDa subunit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
MedChem Express
NFE2 Antibody (YA1209)HY-P81464
TargetNFE2
  • SizePrice
Product group Antibodies
References
GeneTex
NF-E2 antibody [N1C3]GTX102698
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
TargetNFE2
  • SizePrice
Immunohistochemistry analysis in human bone marrow and liver tissues using HPA001914 antibody. Corresponding NFE2 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-NFE2 AntibodyHPA001914
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetNFE2
  • SizePrice
Product group Antibodies
Cusabio
NFE2 AntibodyCSB-PA015750GA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetNFE2
  • SizePrice
Product group Antibodies
Biosynth
NFE2 antibody70R-18854
  • SizePrice
Figure 1. Western blot analysis of NFE2 using anti-NFE2 antibody (A03765-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFE2 antigen affinity purified polyclonal antibody (Catalog # A03765-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFE2 at approximately 41 kDa. The expected band size for NFE2 is at 41 kDa.
Product group Antibodies
Boster Bio
Anti-NFE2 Antibody Picoband(r)A03765-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetNFE2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
NFE2 / p45 AntibodyLS-C667138
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetNFE2
  • SizePrice