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ProductsBack/NFkB p65 antibody
NFkB p65 antibody detects NFkB p65 protein at cytoplasm, nucleus and endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
NFkB p65 antibody detects NFkB p65 protein at cytoplasm, nucleus and endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
NFkB p65 antibody detects NFkB p65 protein at cytoplasm, nucleus and endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).

NFkB p65 antibody

GTX107678
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRELA
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Overview

  • Supplier
    GeneTex
  • Product Name
    NFkB p65 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.49 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5970
  • Target name
    RELA
  • Target description
    RELA proto-oncogene, NF-kB subunit
  • Target synonyms
    AIF3BL3, CMCU, NFKB3, p65, transcription factor p65, NF-kappa-B p65delta3, NF-kappa-B transcription factor p65, nuclear factor NF-kappa-B p65 subunit, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, v-rel avian reticuloendotheliosis viral oncogene homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04206
  • Protein Name
    Transcription factor p65
  • Scientific Description
    NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Chi JY, Hsiao YW, Liang HY, et al. Blockade of the pentraxin 3/CD44 interaction attenuates lung injury-induced fibrosis. Clin Transl Med. 2022,12(11):e1099. doi: 10.1002/ctm2.1099
    Read this paper
  • Pasula S, Gopalakrishnan J, Fu Y, et al. Systemic lupus erythematosus variants modulate the function of an enhancer upstream of TNFAIP3. Front Genet. 2022,13:1011965. doi: 10.3389/fgene.2022.1011965
    Read this paper
  • Qiu W, Chen B, Tang L, et al. Antibiotic Chlortetracycline Causes Transgenerational Immunosuppression via NF-κB. Environ Sci Technol. 2022,56(7):4251-4261. doi: 10.1021/acs.est.1c07343
    Read this paper
  • Bankole O, Scambi I, Parrella E, et al. Beneficial and Sexually Dimorphic Response to Combined HDAC Inhibitor Valproate and AMPK/SIRT1 Pathway Activator Resveratrol in the Treatment of ALS Mice. Int J Mol Sci. 2022,23(3). doi: 10.3390/ijms23031047
    Read this paper
  • Li X, Qian Y, Tang K, et al. Inhibition of lncRNA H19/miR-370-3p pathway mitigates neuronal apoptosis in an in vitro model of spinal cord injury (SCI). Transl Neurosci. 2021,12(1):103-113. doi: 10.1515/tnsci-2021-0013
    Read this paper
  • Chu J, Zhou X, Peng M, et al. Huoxin Pill Attenuates Cardiac Inflammation by Suppression of TLR4/NF-κB in Acute Myocardial Ischemia Injury Rats. Evid Based Complement Alternat Med. 2020,2020:7905902. doi: 10.1155/2020/7905902
    Read this paper
  • Wu CS, Lin SC, Li S, et al. Phloretin alleviates dinitrochlorobenzene-induced dermatitis in BALB/c mice. Int J Immunopathol Pharmacol. 2020,34:2058738420929442. doi: 10.1177/2058738420929442
    Read this paper
  • Shang S, Ji X, Zhang L, et al. Macrophage ABHD5 Suppresses NFκB-Dependent Matrix Metalloproteinase Expression and Cancer Metastasis. Cancer Res. 2019,79(21):5513-5526. doi: 10.1158/0008-5472.CAN-19-1059
    Read this paper
  • Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8
    Read this paper
  • Schiaffino L, Bonafede R, Scambi I, et al. Acetylation state of RelA modulated by epigenetic drugs prolongs survival and induces a neuroprotective effect on ALS murine model. Sci Rep. 2018,8(1):12875. doi: 10.1038/s41598-018-30659-4
    Read this paper

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Figure 1. Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse HEPA1/6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65 at approximately 65-70 kDa. The expected band size for NF-kB p65 is at 60 kDa.
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