IHC-P analysis of mouse spleen tissue using GTX17575 NFkB p65 (phospho Ser529) antibody. Right : Primary antibody Left : a negative control without primary antibody Dilution : 1:50 overnight at 4oC Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 mins
![WB analysis of Jurkat cells unstimulated (lane 1) or stimulated with PMA and Ca2+ (lane 2-5) using GTX17575 NFkB p65 (phospho Ser529) antibody. Lane 1 and 2 : no peptide Lane 3 : non-phospho peptide Lane 4 : generic [pS] peptide Lane 5 : phosphopeptide immunogen](https://www.genetex.com/upload/website/prouct_img/normal/GTX17575/GTX17575_20180525_WB_w_23060620_879.webp "WB analysis of Jurkat cells unstimulated (lane 1) or stimulated with PMA and Ca2+ (lane 2-5) using GTX17575 NFkB p65 (phospho Ser529) antibody. Lane 1 and 2 : no peptide Lane 3 : non-phospho peptide Lane 4 : generic [pS] peptide Lane 5 : phosphopeptide immunogen")
IHC-P analysis of mouse spleen tissue using GTX17575 NFkB p65 (phospho Ser529) antibody. Right : Primary antibody Left : a negative control without primary antibody Dilution : 1:50 overnight at 4oC Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 mins
NFkB p65 (phospho Ser529) antibody
GTX17575
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetRELA
Overview
- SupplierGeneTex
- Product NameNFkB p65 (phospho Ser529) antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID5970
- Target nameRELA
- Target descriptionRELA proto-oncogene, NF-kB subunit
- Target synonymsCMCU; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFKB3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDQ04206
- Protein NameTranscription factor p65
- Scientific DescriptionNF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203