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ProductsBack/NGFR / CD271 / TNR16 Antibody

NGFR / CD271 / TNR16 Antibody

LS-C812719
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetNGFR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NGFR / CD271 / TNR16 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), IF (1:200 - 1:1000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4804
  • Target name
    NGFR
  • Target description
    nerve growth factor receptor
  • Target synonyms
    CD271; Gp80-LNGFR; low affinity neurotrophin receptor p75NTR; low-affinity nerve growth factor receptor; NGF receptor; p75 ICD; p75(NTR); p75NTR; TNFR superfamily, member 16; TNFRSF16; tumor necrosis factor receptor superfamily member 16
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of p75 NGF Receptor/NGFR using anti-p75 NGF Receptor/NGFR antibody (A01187). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: human Sw620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p75 NGF Receptor/NGFR antigen affinity purified polyclonal antibody (Catalog # A01187) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p75 NGF Receptor/NGFR at approximately 45KD,75KD. The expected band size for p75 NGF Receptor/NGFR is at 45KD,75KD.
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