NOTCH1 antibody

GeneTex

NOTCH1 antibody

9

WB: 1:500-1:2000. IHC-P: 1:200. ELISA: 1:20000-1:60000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Dot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, Other Application

Research Use Only

Polyclonal

90 mg/ml

Unconjugated

NOTCH1

notch receptor 1

AOS5, AOVD1, TAN1, hN1, neurogenic locus notch homolog protein 1, Notch homolog 1, translocation-associated, notch 1, translocation-associated notch protein TAN-1

Rabbit

IgG

Neurogenic locus notch homolog protein 1

Notch 1 is synthesized in the endoplasmic reticulum as an inactive form, which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

-20°C or -80°C,2°C to 8°C

12352203