
IHC-P analysis of exocrine glands of human pancreas using Rabbit anti-Notch1 antibody (GTX28925) at a dilution of 1:200. Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.
NOTCH1 antibody
GTX28925
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, Other Application
Product group Antibodies
TargetNOTCH1
Overview
- SupplierGeneTex
- Product NameNOTCH1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. IHC-P: 1:200. ELISA: 1:20000-1:60000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration90 mg/ml
- ConjugateUnconjugated
- Gene ID4851
- Target nameNOTCH1
- Target descriptionnotch receptor 1
- Target synonymsAOS5; AOVD1; hN1; neurogenic locus notch homolog protein 1; notch 1; Notch homolog 1, translocation-associated; TAN1; translocation-associated notch protein TAN-1
- HostRabbit
- IsotypeIgG
- Protein IDP46531
- Protein NameNeurogenic locus notch homolog protein 1
- Scientific DescriptionNotch 1 is synthesized in the endoplasmic reticulum as an inactive form, which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- MicroRNA-223-induced inhibition of the FBXW7 gene affects the proliferation and apoptosis of colorectal cancer cells via the Notch and Akt/mTOR pathways. Liu Z et al., 2021 Feb, Mol Med RepRead more
- Role of stromal cell-mediated Notch signaling in CLL resistance to chemotherapy. Nwabo Kamdje AH et al., 2012 May, Blood Cancer JRead more
- Notch 1 signaling pathway effect on implantation competency. Chu PW et al., 2011 Nov, Fertil SterilRead more
- Numb mediates the interaction between Wnt and Notch to modulate primitive erythropoietic specification from the hemangioblast. Cheng X et al., 2008 Oct, DevelopmentRead more