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Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: NOVA1 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 20, 50, 52 kDa Observed band size: 53, 50 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: NOVA1 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 20, 50, 52 kDa Observed band size: 53, 50 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: NOVA1 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 20, 50, 52 kDa Observed band size: 53, 50 kDa

NOVA1 Antibody

CSB-PA015957LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNOVA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NOVA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4857
  • Target name
    NOVA1
  • Target description
    NOVA alternative splicing regulator 1
  • Target synonyms
    neuro-oncological ventral antigen 1; Nova-1; onconeural ventral antigen 1; paraneoplastic Ri antigen; RNA-binding protein Nova-1; ventral neuron-specific protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51513
  • Protein Name
    RNA-binding protein Nova-1
  • Scientific Description
    May regulate RNA splicing or metabolism in a specific subset of developing neurons.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nova1) Polyclonal Antibody, Unconjugated (bs-11324R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Figure 1. Western blot analysis of Nova1 using anti-Nova1 antibody (A06087-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nova1 antigen affinity purified polyclonal antibody (Catalog # A06087-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nova1 at approximately 52-75 kDa. The expected band size for Nova1 is at 52 kDa.
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