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Nova1 Antibody (YA1421, Rabbit)

HY-P81676
MedChem Express
TargetNOVA1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Nova1 Antibody (YA1421, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4857
  • Target name
    NOVA1
  • Target description
    NOVA alternative splicing regulator 1
  • Target synonyms
    Nova-1, RNA-binding protein Nova-1, neuro-oncological ventral antigen 1, onconeural ventral antigen 1, paraneoplastic Ri antigen, ventral neuron-specific protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Nova1 Antibody (YA1421) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1421), targeting Nova1, with a predicted molecular weight of 52 kDa (observed band size: 52 kDa). Nova1 Antibody (YA1421) can be used for WB, IHC-P experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Nova1 using anti-Nova1 antibody (A06087-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nova1 antigen affinity purified polyclonal antibody (Catalog # A06087-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nova1 at approximately 52-75 kDa. The expected band size for Nova1 is at 52 kDa.
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