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NOVA1 antibody

GTX102831
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetNOVA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    NOVA1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.92 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4857
  • Target name
    NOVA1
  • Target description
    NOVA alternative splicing regulator 1
  • Target synonyms
    neuro-oncological ventral antigen 1; Nova-1; onconeural ventral antigen 1; paraneoplastic Ri antigen; RNA-binding protein Nova-1; ventral neuron-specific protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51513
  • Protein Name
    RNA-binding protein Nova-1
  • Scientific Description
    This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nova1 using anti-Nova1 antibody (A06087-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nova1 antigen affinity purified polyclonal antibody (Catalog # A06087-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nova1 at approximately 52-75 kDa. The expected band size for Nova1 is at 52 kDa.
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