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Western Blot analysis of Jurkat cells using NQO1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using NQO1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using NQO1 Polyclonal Antibody

NQO1 Antibody

CSB-PA003475
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNQO1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NQO1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1728
  • Target name
    NQO1
  • Target description
    NAD(P)H quinone dehydrogenase 1
  • Target synonyms
    DHQU, DIA4, DTD, NMOR1, NMORI, QR1, NAD(P)H dehydrogenase [quinone] 1, DT-diaphorase, NAD(P)H dehydrogenase, quinone 1, NAD(P)H-quinone oxidoreductase, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H:menadione oxidoreductase 1, NAD(P)H:quinone oxidoreductase 1, NAD(P)H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH) (cytochrome b-5 reductase), diaphorase-4, dioxin-inducible 1, menadione reductase, phylloquinone reductase, quinone reductase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15559
  • Protein Name
    NAD(P)H dehydrogenase [quinone] 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NQO1 using anti-NQO1 antibody (A00494-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO1 antigen affinity purified polyclonal antibody (Catalog # A00494-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NQO1 at approximately 31 kDa. The expected band size for NQO1 is at 31 kDa.
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