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Formalin-fixed and paraffin embedded mouse colon carcinoma labeled with Anti-NQO1 Polyclonal Antibody, Unconjugated (bs-2184R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse colon carcinoma labeled with Anti-NQO1 Polyclonal Antibody, Unconjugated (bs-2184R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse colon carcinoma labeled with Anti-NQO1 Polyclonal Antibody, Unconjugated (bs-2184R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

NQO1 Polyclonal Antibody

BS-2184R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNQO1
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Overview

  • Supplier
    Bioss
  • Product Name
    NQO1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1728
  • Target name
    NQO1
  • Target description
    NAD(P)H quinone dehydrogenase 1
  • Target synonyms
    DHQU, DIA4, DTD, NMOR1, NMORI, QR1, NAD(P)H dehydrogenase [quinone] 1, DT-diaphorase, NAD(P)H dehydrogenase, quinone 1, NAD(P)H-quinone oxidoreductase, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H:menadione oxidoreductase 1, NAD(P)H:quinone oxidoreductase 1, NAD(P)H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH) (cytochrome b-5 reductase), diaphorase-4, dioxin-inducible 1, menadione reductase, phylloquinone reductase, quinone reductase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15559
  • Protein Name
    NAD(P)H dehydrogenase [quinone] 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wang X, Wang Z, Wu H, et al. Sarcodon imbricatus polysaccharides protect against cyclophosphamide-induced immunosuppression via regulating Nrf2-mediated oxidative stress. Int J Biol Macromol. 2018,120(Pt A):736-744. doi: 10.1016/j.ijbiomac.2018.08.157
    Read this paper
  • Zhang C, Wang F, Xie Z, et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. Oncotarget. 2018,9(2):2175-2192. doi: 10.18632/oncotarget.23299
    Read this paper
  • Zhang Z, Qu J, Zheng C, et al. Nrf2 antioxidant pathway suppresses Numb-mediated epithelial-mesenchymal transition during pulmonary fibrosis. Cell Death Dis. 2018,9(2):83. doi: 10.1038/s41419-017-0198-x
    Read this paper
  • Shen H, Wu N, Wang Y, et al. Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis. Int Immunopharmacol. 2017,46:16-22. doi: 10.1016/j.intimp.2017.02.020
    Read this paper
  • Tang H, Gao L, Mao J, et al. Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways. Cell Stress Chaperones. 2016,21(2):239-49. doi: 10.1007/s12192-015-0654-4
    Read this paper

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Figure 1. Western blot analysis of NQO1 using anti-NQO1 antibody (A00494-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO1 antigen affinity purified polyclonal antibody (Catalog # A00494-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NQO1 at approximately 31 kDa. The expected band size for NQO1 is at 31 kDa.
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