Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NRF1 antibody [HL3308] (GTX641070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![HepG2 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NRF1 antibody [HL3308] (GTX641070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641070/GTX641070_T-45544_20240927_WB_Fraction_24100318_541.webp "HepG2 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NRF1 antibody [HL3308] (GTX641070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![NRF1 antibody [HL3308] detects NRF1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF1 stained by NRF1 antibody [HL3308] (GTX641070) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641070/GTX641070_T-45544_20241011_ICC_IF_24110700_984.webp "NRF1 antibody [HL3308] detects NRF1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF1 stained by NRF1 antibody [HL3308] (GTX641070) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.")
![Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NRF1 antibody [HL3308] (GTX641070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641070/GTX641070_45600_20250124_WB_Z_25020422_962.webp "Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NRF1 antibody [HL3308] (GTX641070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NRF1 antibody [HL3308] (GTX641070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NRF1 antibody [HL3308]
GTX641070
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetNRF1
Overview
- SupplierGeneTex
- Product NameNRF1 antibody [HL3308]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3308
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4899
- Target nameNRF1
- Target descriptionnuclear respiratory factor 1
- Target synonymsALPHA-PAL, nuclear respiratory factor 1, alpha palindromic-binding protein
- HostRabbit
- IsotypeIgG
- Protein IDQ16656
- Protein NameNuclear respiratory factor 1
- Scientific DescriptionThis gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for nuclear factor (erythroid-derived 2)-like 1 which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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