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NUBPL Antibody

LS-C816846
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
TargetNUBPL
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NUBPL Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:100 - 1:500), IF (1:100 - 1:500) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID80224
  • Target name
    NUBPL
  • Target description
    NUBP iron-sulfur cluster assembly factor, mitochondrial
  • Target synonyms
    C14orf127, IND1, MC1DN21, huInd1, iron-sulfur cluster transfer protein NUBPL, IND1 homolog, iron-sulfur protein required for NADH dehydrogenase, nucleotide binding protein like
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUBPL using anti-NUBPL antibody (A10634-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Colo320 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUBPL antigen affinity purified polyclonal antibody (Catalog # A10634-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUBPL at approximately 34 kDa. The expected band size for NUBPL is at 34 kDa.
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