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WB analysis of rat spleen tissue lysate using GTX66231 NUDT15 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of rat spleen tissue lysate using GTX66231 NUDT15 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of rat spleen tissue lysate using GTX66231 NUDT15 antibody. Dilution : 1:1000 Loading : 25microg per lane

NUDT15 antibody

GTX66231
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetNUDT15
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Overview

  • Supplier
    GeneTex
  • Product Name
    NUDT15 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55270
  • Target name
    NUDT15
  • Target description
    nudix hydrolase 15
  • Target synonyms
    8-oxo-dGTPase NUDT15; MTH2; mutT homolog 2; nucleoside diphosphate-linked to another moiety X hydrolase 15; nucleotide triphosphate diphosphatase NUDT15; nudix (nucleoside diphosphate linked moiety X)-type motif 15; NUDT15D; probable 7,8-dihydro-8-oxoguanine triphosphatase NUDT15; probable 8-oxo-dGTP diphosphatase NUDT15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NV35
  • Protein Name
    Nucleotide triphosphate diphosphatase NUDT15
  • Scientific Description
    This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Apr 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUDT15 using anti-NUDT15 antibody (A05498-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT15 antigen affinity purified polyclonal antibody (Catalog # A05498-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT15 at approximately 19 kDa. The expected band size for NUDT15 is at 19 kDa.
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