![IHC-P analysis of human endometrium tissue using GTX83850 Oct4 antibody [9B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human endometrium tissue using GTX83850 Oct4 antibody [9B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83850/GTX83850_1927_IHC-P_w_23061420_711.webp)
IHC-P analysis of human endometrium tissue using GTX83850 Oct4 antibody [9B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
Oct4 antibody [9B7]
GTX83850
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPOU5F1
Overview
- SupplierGeneTex
- Product NameOct4 antibody [9B7] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000. ICC/IF: 1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9B7
- Concentration0.25 mg/ml
- ConjugateUnconjugated
- Gene ID5460
- Target namePOU5F1
- Target descriptionPOU class 5 homeobox 1
- Target synonymsOCT3, OCT4, OCT4Borf1, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4, POU domain, class 5, transcription factor 1, POU domain transcription factor OCT4, POU-type homeodomain-containing DNA-binding protein, octamer-binding protein 3, octamer-binding protein 4, octamer-binding transcription factor 3
- HostMouse
- IsotypeIgG1
- Protein IDQ01860
- Protein NamePOU domain, class 5, transcription factor 1
- Scientific DescriptionTranscription factor that binds to the octamer motif (5-ATTTGCAT-3). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of human lung tissue using GTX83850 Oct4 antibody [9B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human lung tissue using GTX83850 Oct4 antibody [9B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83850/GTX83850_1928_IHC-P_w_23061420_874.webp)
![IHC-P analysis of human ovary tissue using GTX83850 Oct4 antibody [9B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human ovary tissue using GTX83850 Oct4 antibody [9B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83850/GTX83850_1929_IHC-P_w_23061420_531.webp)
![WB analysis of MDCK cell lysate using GTX83850 Oct4 antibody [9B7]. Loading : 10 ug per lane Dilution : 1:200 WB analysis of MDCK cell lysate using GTX83850 Oct4 antibody [9B7]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83850/GTX83850_3462_WB_w_23061420_228.webp)
![WB analysis of HEK293T cells transfected with Oct4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83850 Oct4 antibody [9B7]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with Oct4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83850 Oct4 antibody [9B7]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83850/GTX83850_3951_WB_w_23061420_712.webp)
![ICC/IF analysis of COS7 cells transiently transfected with 43377 plasmid using GTX83850 Oct4 antibody [9B7]. ICC/IF analysis of COS7 cells transiently transfected with 43377 plasmid using GTX83850 Oct4 antibody [9B7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83850/GTX83850_850_ICCIF_w_23061420_306.webp)



![IHC-P analysis of human testes, seminoma tissue using GTX01931 Oct4 antibody [N1NK]. Note nuclear staining of neoplastic cells.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01931/GTX01931_20200811_IHC-P_65_w_23053121_845.webp)

![FACS analysis of Jurkat cells using GTX82755 Oct4 antibody [7E7]. Green : Oct4 Purple : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX82755/GTX82755_20170912_FACS_w_23061322_841.webp)
![WB analysis of PMA treated HepG2 cell lysate using GTX83020 Oct4 antibody [3A5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83020/GTX83020_20170912_WB_w_23061322_182.webp)