Bio-Connect

Oct4 antibody [GT486]

GTX627419
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPOU5F1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Oct4 antibody [GT486]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:1000-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT486
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5460
  • Target name
    POU5F1
  • Target description
    POU class 5 homeobox 1
  • Target synonyms
    OCT3, OCT4, OCT4Borf1, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4, POU domain, class 5, transcription factor 1, POU domain transcription factor OCT4, POU-type homeodomain-containing DNA-binding protein, octamer-binding protein 3, octamer-binding protein 4, octamer-binding transcription factor 3
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ01860
  • Protein Name
    POU domain, class 5, transcription factor 1
  • Scientific Description
    This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewings sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Masola V, Franchi M, Zaza G, et al. Heparanase regulates EMT and cancer stem cell properties in prostate tumors. Front Oncol. 2022,12:918419. doi: 10.3389/fonc.2022.918419
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  • Mashiko T, Kanayama K, Saito N, et al. Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres. Cells. 2021,10(3). doi: 10.3390/cells10030560
    Read this paper
  • Lee KY, Kuo TC, Chou CM, et al. Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling. Cells. 2020,10(1). doi: 10.3390/cells10010028
    Read this paper
  • Andreucci E, Peppicelli S, Ruzzolini J, et al. The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells. J Mol Med (Berl). 2020,98(10):1431-1446. doi: 10.1007/s00109-020-01959-y
    Read this paper
  • Ramírez-Rentería C, Espinosa-De-Los-Monteros AL, Etual EC, et al. From ACTH-Dependent to ACTH-Independent Cushing's Syndrome from a Malignant Mixed Corticomedullary Adrenal Tumor: Potential Role of Embryonic Stem Cells. Case Rep Endocrinol. 2020,2020:4768281. doi: 10.1155/2020/4768281
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  • Aminuddin A, Ng PY, Leong CO, et al. Mitochondrial DNA alterations may influence the cisplatin responsiveness of oral squamous cell carcinoma. Sci Rep. 2020,10(1):7885. doi: 10.1038/s41598-020-64664-3
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  • Wang X, Xie S, Li Z, et al. Generation of an iPSC line (HUSTi002-A) from fibroblasts of a patient with Sertoli cell-only syndrome carrying c.731_732delAT in PIWIL2 gene. Stem Cell Res. 2020,42:101703. doi: 10.1016/j.scr.2020.101703
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  • Chen YL, Liu KJ, Jang CW, et al. ERK Activation Modulates Cancer Stemness and Motility of a Novel Mouse Oral Squamous Cell Carcinoma Cell Line. Cancers (Basel). 2019,12(1). doi: 10.3390/cancers12010061
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  • Huang TH, Wu ATH, Cheng TS, et al. In silico identification of thiostrepton as an inhibitor of cancer stem cell growth and an enhancer for chemotherapy in non-small-cell lung cancer. J Cell Mol Med. 2019,23(12):8184-8195. doi: 10.1111/jcmm.14689
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  • Chien CH, Chuang JY, Yang ST, et al. Enrichment of superoxide dismutase 2 in glioblastoma confers to acquisition of temozolomide resistance that is associated with tumor-initiating cell subsets. J Biomed Sci. 2019,26(1):77. doi: 10.1186/s12929-019-0565-2
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