![Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT735] (GTX627423) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT735] (GTX627423) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX627423/GTX627423_40980_20210723_WB_shRNA_watermark_w_23061202_871.webp)
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT735] (GTX627423) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Oct4 antibody [GT735]
GTX627423
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPOU5F1
Overview
- SupplierGeneTex
- Product NameOct4 antibody [GT735]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT735
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5460
- Target namePOU5F1
- Target descriptionPOU class 5 homeobox 1
- Target synonymsOCT3, OCT4, OCT4Borf1, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4, POU domain, class 5, transcription factor 1, POU domain transcription factor OCT4, POU-type homeodomain-containing DNA-binding protein, octamer-binding protein 3, octamer-binding protein 4, octamer-binding transcription factor 3
- HostMouse
- IsotypeIgG1
- Protein IDQ01860
- Protein NamePOU domain, class 5, transcription factor 1
- Scientific DescriptionThis gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewings sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203

![Oct4 antibody [GT735] detects Oct4 protein by Western blot analysis. A. 30 μg human ESC whole cell lysate/extract 10 % SDS-PAGE (GTX627423) dilution: 1:1000 Oct4 antibody [GT735] detects Oct4 protein by Western blot analysis. A. 30 μg human ESC whole cell lysate/extract 10 % SDS-PAGE (GTX627423) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX627423/GTX627423_40980_WB_hESC_w_23061202_796.webp)
![Oct4 antibody [GT735] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT735] dilution: 1:50 Oct4 antibody [GT735] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT735] dilution: 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX627423/GTX627423_40980_CT_FACS_w_23061202_205.webp)








