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p27 Kip1 antibody

GTX14243
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetCDKN1B
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Overview

  • Supplier
    GeneTex
  • Product Name
    p27 Kip1 antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1027
  • Target name
    CDKN1B
  • Target description
    cyclin dependent kinase inhibitor 1B
  • Target synonyms
    CDKN4; cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); KIP1; MEN1B; MEN4; P27KIP1
  • Host
    Chicken
  • Isotype
    IgY
  • Protein IDP46527
  • Protein Name
    Cyclin-dependent kinase inhibitor 1B
  • Scientific Description
    Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up-regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot using GeneTex affinity purified anti-p27 antibody (GTX26547) shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of Dylight? 800 conjugated Goat anti Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.
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The data was published in the journal Mol Cell Endocrinol in 2013.PMID: 23791816
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