More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/p38 MAPK (phospho Thr180/Tyr182) antibody
Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK (phospho Thr180/Tyr182) antibody (GTX133460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK (phospho Thr180/Tyr182) antibody (GTX133460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK (phospho Thr180/Tyr182) antibody (GTX133460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

p38 MAPK (phospho Thr180/Tyr182) antibody

GTX133460
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAPK14
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    p38 MAPK (phospho Thr180/Tyr182) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1432
  • Target name
    MAPK14
  • Target description
    mitogen-activated protein kinase 14
  • Target synonyms
    CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA, mitogen-activated protein kinase 14, CSAID-binding protein, MAP kinase 14, MAP kinase Mxi2, MAP kinase p38 alpha, MAX-interacting protein 2, cytokine suppressive anti-inflammatory drug binding protein, mitogen-activated protein kinase p38 alpha, p38 MAP kinase, p38 mitogen activated protein kinase, p38alpha Exip, stress-activated protein kinase 2A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16539
  • Protein Name
    Mitogen-activated protein kinase 14
  • Scientific Description
    The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Lee SC, Shen CY, Wang WH, et al. Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model. Pharmaceuticals (Basel). 2023,16(9). doi: 10.3390/ph16091188
    Read this paper
  • Zhang X, Lian X, Li H, et al. Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis. Chin Herb Med. 2022,14(4):554-562. doi: 10.1016/j.chmed.2021.03.002
    Read this paper
  • Fu L, Shi Z, Chen B. Deleted in lymphocytic leukemia 2 induces retinoic acid receptor beta promoter methylation and mitogen activated kinase-like protein activation to enhance viability and mobility of colorectal cancer cells. Bioengineered. 2022,13(5):12847-12862. doi: 10.1080/21655979.2022.2076482
    Read this paper
  • Qian X, Zhang Y. EZH2 enhances proliferation and migration of trophoblast cell lines by blocking GADD45A-mediated p38/MAPK signaling pathway. Bioengineered. 2022,13(5):12583-12597. doi: 10.1080/21655979.2022.2074620
    Read this paper
  • Vadana M, Cecoltan S, Ciortan L, et al. Parathyroid Hormone Induces Human Valvular Endothelial Cells Dysfunction That Impacts the Osteogenic Phenotype of Valvular Interstitial Cells. Int J Mol Sci. 2022,23(7). doi: 10.3390/ijms23073776
    Read this paper
  • Usuki S, Tamura N, Tamura T, et al. Konjac Ceramide (kCer)-Mediated Signal Transduction of the Sema3A Pathway Promotes HaCaT Keratinocyte Differentiation. Biology (Basel). 2022,11(1). doi: 10.3390/biology11010121
    Read this paper
  • Li Y, Lv X, Jiang M, et al. Sitagliptin ameliorates hypoxia-induced damages in endometrial stromal cells: an implication in endometriosis. Bioengineered. 2022,13(1):800-809. doi: 10.1080/21655979.2021.2012950
    Read this paper
  • Liu JF, Chi MC, Lin CY, et al. PM2.5 facilitates IL-6 production in human osteoarthritis synovial fibroblasts via ASK1 activation. J Cell Physiol. 2021,236(3):2205-2213. doi: 10.1002/jcp.30009
    Read this paper
  • Rocha-Neto LM, Gamarra-Suárez JR, Freitas FF, et al. Early Phase of Type 1 Diabetes Decreases the Responsiveness of C-Fiber Nociceptors in the Temporomandibular Joint of Rats. Neuroscience. 2019,416:229-238. doi: 10.1016/j.neuroscience.2019.08.011
    Read this paper
  • Li HY, Leu YL, Wu YC, et al. Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice. J Agric Food Chem. 2019,67(7):1889-1901. doi: 10.1021/acs.jafc.8b06217
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Biosynth
P38 MAPK antibody20R-2403
  • SizePrice
Product group Antibodies
RayBiotech
Anti-p38 MAPK Antibody144-62044
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPK14
  • SizePrice
Figure 1. Western blot analysis of P38 Alpha/MAPK14 using anti-P38 Alpha/MAPK14 antibody (A00176-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P38 Alpha/MAPK14 antigen affinity purified polyclonal antibody (Catalog # A00176-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P38 Alpha/MAPK14 at approximately 41 kDa. The expected band size for P38 Alpha/MAPK14 is at 41 kDa.
Product group Antibodies
Boster Bio
Anti-p38 alpha/MAPK14 Antibody Picoband(r)A00176-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK14
  • SizePrice
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK (phospho Thr180/Tyr182) antibody (GTX130383) diluted at 1:500.
Product group Antibodies
GeneTex
p38 MAPK (phospho Thr180/Tyr182) antibodyGTX130383
ApplicationsWestern Blot
ReactivityHuman
TargetMAPK14
  • SizePrice
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK (phospho Tyr182) antibody (GTX133881) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
p38 MAPK (phospho Tyr182) antibodyGTX133881
ApplicationsWestern Blot
ReactivityHuman
TargetMAPK14
  • SizePrice
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody (GTX110720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
p38 MAPK antibody [N1C3-2]GTX110720
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Human, Mouse, Rat
TargetMAPK14
  • SizePrice
ELISA analysis of antigen using GTX60771 p38 MAPK antibody [8G4D11]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
Product group Antibodies
GeneTex
p38 MAPK antibody [8G4D11]GTX60771
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Monkey, Mouse, Rat
TargetMAPK14
  • SizePrice
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [HL1006] (GTX635797) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
p38 MAPK antibody [HL1006]GTX635797
ApplicationsWestern Blot
ReactivityCanine, Feline, Human, Mouse, Rat
TargetMAPK14
  • SizePrice
WB analysis of Jurkat cells using GTX77721 p38 MAPK antibody at 0.625μg/ml.
Product group Antibodies
References
GeneTex
p38 MAPK antibody, C-termGTX77721
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetMAPK14
  • SizePrice
IHC-P analysis of human breast carcinoma tissue using GTX78989 p38 MAPK (phospho Tyr182) antibody.
Product group Antibodies
GeneTex
p38 MAPK (phospho Tyr182) antibodyGTX78989
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Monkey, Mouse
TargetMAPK14
  • SizePrice