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ProductsBack/p38 (MAPK14) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2F2]
Detection of endogenous Mxi / SAPK2alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Mxi-2F2 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mab Mxi-2F2 recognizes SAPK2a/p38 at 38 kDa and its splice variant Mxi2 at 30 kDa. lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA231; lane 7: T47D
Detection of endogenous Mxi / SAPK2alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Mxi-2F2 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mab Mxi-2F2 recognizes SAPK2a/p38 at 38 kDa and its splice variant Mxi2 at 30 kDa. lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA231; lane 7: T47D
Detection of endogenous Mxi / SAPK2alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Mxi-2F2 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mab Mxi-2F2 recognizes SAPK2a/p38 at 38 kDa and its splice variant Mxi2 at 30 kDa. lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA231; lane 7: T47D

p38 (MAPK14) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2F2]

AM00097PU-N
OriGene
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetMAPK14
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    p38 (MAPK14) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2F2]
  • Delivery Days Customer
    14
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2F2
  • Gene ID1432
  • Target name
    MAPK14
  • Target description
    mitogen-activated protein kinase 14
  • Target synonyms
    CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA, mitogen-activated protein kinase 14, CSAID-binding protein, MAP kinase 14, MAP kinase Mxi2, MAP kinase p38 alpha, MAX-interacting protein 2, cytokine suppressive anti-inflammatory drug binding protein, mitogen-activated protein kinase p38 alpha, p38 MAP kinase, p38 mitogen activated protein kinase, p38alpha Exip, stress-activated protein kinase 2A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ16539
  • Protein Name
    Mitogen-activated protein kinase 14
  • Scientific Description
    p38 (MAPK14) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 2F2, Purified
  • Storage Instruction
    -20°C,-80°C,2°C to 8°C
  • UNSPSC
    12352203

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References
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Figure 1. Western blot analysis of P38 Alpha/MAPK14 using anti-P38 Alpha/MAPK14 antibody (A00176-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P38 Alpha/MAPK14 antigen affinity purified polyclonal antibody (Catalog # A00176-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P38 Alpha/MAPK14 at approximately 41 kDa. The expected band size for P38 Alpha/MAPK14 is at 41 kDa.
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Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-p38 MAPK (Thr180/Tyr182) RM243 at a 1:1000 dilution.
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