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PACSIN2 Antibody

LS-C409330
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPACSIN2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PACSIN2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 51kDa/55kDa, while the observed MW by Western blot was 56kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 51kDa/55kDa, while the observed MW by Western blot was 56kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11252
  • Target name
    PACSIN2
  • Target description
    protein kinase C and casein kinase substrate in neurons 2
  • Target synonyms
    SDPII, protein kinase C and casein kinase substrate in neurons protein 2, cytoplasmic phosphoprotein PACSIN2, syndapin-2, syndapin-II, syndapin2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA793216(PACSIN2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of PACSIN2 using anti-PACSIN2 antibody (A04211-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat heart tissue tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACSIN2 antigen affinity purified polyclonal antibody (Catalog # A04211-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PACSIN2 at approximately 60 kDa. The expected band size for PACSIN2 is at 56,60-65 kDa.
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