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PACSIN2 antibody [N2C3]

GTX104204
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPACSIN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    PACSIN2 antibody [N2C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID11252
  • Target name
    PACSIN2
  • Target description
    protein kinase C and casein kinase substrate in neurons 2
  • Target synonyms
    SDPII, protein kinase C and casein kinase substrate in neurons protein 2, cytoplasmic phosphoprotein PACSIN2, syndapin-2, syndapin-II, syndapin2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNF0
  • Protein Name
    Protein kinase C and casein kinase substrate in neurons protein 2
  • Scientific Description
    This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation by regulating tubulin polymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Popov S, Popova E, Inoue M, et al. HIV-1 gag recruits PACSIN2 to promote virus spreading. Proc Natl Acad Sci U S A. 2018,115(27):7093-7098. doi: 10.1073/pnas.1801849115
    Read this paper
  • Karbalaei MS, Rippe C, Albinsson S, et al. Impaired contractility and detrusor hypertrophy in cavin-1-deficient mice. Eur J Pharmacol. 2012,689(1-3):179-85. doi: 10.1016/j.ejphar.2012.05.023
    Read this paper

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Figure 1. Western blot analysis of PACSIN2 using anti-PACSIN2 antibody (A04211-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat heart tissue tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACSIN2 antigen affinity purified polyclonal antibody (Catalog # A04211-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PACSIN2 at approximately 60 kDa. The expected band size for PACSIN2 is at 56,60-65 kDa.
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