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PACSIN2 Polyclonal Antibody

CAC15670
Biomatik
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPACSIN2
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Overview

  • Supplier
    Biomatik
  • Product Name
    PACSIN2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11252
  • Target name
    PACSIN2
  • Target description
    protein kinase C and casein kinase substrate in neurons 2
  • Target synonyms
    cytoplasmic phosphoprotein PACSIN2; protein kinase C and casein kinase substrate in neurons protein 2; SDPII; syndapin2; syndapin-2; syndapin-II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNF0
  • Protein Name
    Protein kinase C and casein kinase substrate in neurons protein 2
  • Scientific Description
    The PACSIN2 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PACSIN2 using anti-PACSIN2 antibody (A04211-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat heart tissue tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACSIN2 antigen affinity purified polyclonal antibody (Catalog # A04211-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PACSIN2 at approximately 60 kDa. The expected band size for PACSIN2 is at 56,60-65 kDa.
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Lane 1: Daudi cell lysates; Lane 2: HL-60 cell lysates; Lane 3: Human brain tissue lysates; Lane 4: Mouse brain tissue lysates probed with PACSIN2 Monoclonal Antibody, Unconjugated (bsm-51639M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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