WB analysis of wild-type (WT) and PARP (cleaved) knockout (KO) HeLa cell extracts using GTX00943 PARP (cleaved) antibody [GT1181]. Dilution : 1:1000 Laoding : 25microg
![WB analysis of Jurkat cell extracts with/without Staurosporine (1microM, RT, 3 hrs) treatment using GTX00943 PARP (cleaved) antibody [GT1181]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00943/GTX00943_20200327_WB_2_w_23053121_314.webp "WB analysis of Jurkat cell extracts with/without Staurosporine (1microM, RT, 3 hrs) treatment using GTX00943 PARP (cleaved) antibody [GT1181]. Dilution : 1:1000 Loading : 25microg")
![WB analysis of mouse spleen tissue and NIH-3T3 cell lysate using GTX00943 PARP (cleaved) antibody [GT1181]. Lane 1 : mouse spleen tissue lysate Lane 2 : NIH-3T3 whole cell lysate Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00943/GTX00943_20200327_WB_3_w_23053121_295.webp "WB analysis of mouse spleen tissue and NIH-3T3 cell lysate using GTX00943 PARP (cleaved) antibody [GT1181]. Lane 1 : mouse spleen tissue lysate Lane 2 : NIH-3T3 whole cell lysate Dilution : 1:1000 Loading : 25microg")
![Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 12% SDS-PAGE, and the membrane was blotted with PARP (cleaved) antibody [GT1181] (GTX00943) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00943/GTX00943_4000000091_20200313_WB_treatment_Cisplatin_w_23053121_796.webp "Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 12% SDS-PAGE, and the membrane was blotted with PARP (cleaved) antibody [GT1181] (GTX00943) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 12% SDS-PAGE, and the membrane was blotted with PARP (cleaved) antibody [GT1181] (GTX00943) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00943/GTX00943_4000000091_20200313_WB_treatment_Staurosporine_w_23053121_913.webp "Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 12% SDS-PAGE, and the membrane was blotted with PARP (cleaved) antibody [GT1181] (GTX00943) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
WB analysis of wild-type (WT) and PARP (cleaved) knockout (KO) HeLa cell extracts using GTX00943 PARP (cleaved) antibody [GT1181]. Dilution : 1:1000 Laoding : 25microg
PARP (cleaved) antibody [GT1181] - Recombinant antibody, KO/KD, Orthogonal Validated
GTX00943
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Porcine
TargetPARP1
Overview
- SupplierGeneTex
- Product NamePARP (cleaved) antibody [GT1181] - Recombinant antibody, KO/KD, Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody is specific for cleaved PARP (~25kDa). It does not recognize full-length (~116kDa) and the large fragment (~89 kDa).
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1181
- ConjugateUnconjugated
- Gene ID142
- Target namePARP1
- Target descriptionpoly(ADP-ribose) polymerase 1
- Target synonymsADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT; ADPRT 1; ADPRT1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; PARP-1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; PPOL; protein poly-ADP-ribosyltransferase PARP1
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThis gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Porcine
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- Expression of Caspases in the Pig Endometrium Throughout the Estrous Cycle and at the Maternal-Conceptus Interface During Pregnancy and Regulation by Steroid Hormones and Cytokines. Jung W et al., 2021, Front Vet SciRead more
- Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo. Wang YY et al., 2020 Mar 31, ACS OmegaRead more
- Apatinib Inhibits Cell Proliferation and Induces Autophagy in Human Papillary Thyroid Carcinoma via the PI3K/Akt/mTOR Signaling Pathway. Meng X et al., 2020, Front OncolRead more