PathPlus(tm) CALR / Calreticulin Antibody (clone 5F12-B10)

LifeSpan BioSciences

PathPlus(tm) CALR / Calreticulin Antibody (clone 5F12-B10)

23

Immunohistochemistry: LS-B3411 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3411 was determined to be 10 ug/ml.. IHC, IHC-P (10 µg/ml), IP (12.5 µg/ml), WB (1:1000, 12.5 µg/ml) Immunohistochemistry: LS-B3411 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3411 was determined to be 10 ug/ml.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

5F12-B10

Unconjugated

CALR

calreticulin

CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR, calreticulin, CRP55, ERp60, HACBP, Sicca syndrome antigen A (autoantigen Ro; calreticulin), calregulin, endoplasmic reticulum resident protein 60, epididymis secretory sperm binding protein Li 99n, grp60

Mouse

IgG1

Human, Monkey

-20°C

41116161