More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PathPlus(tm) CCNE1 / Cyclin E1 Antibody (C-Terminus)

PathPlus(tm) CCNE1 / Cyclin E1 Antibody (C-Terminus)

LS-B3337
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCCNE1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) CCNE1 / Cyclin E1 Antibody (C-Terminus)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: LS-B3337 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3337 was determined to be 10 ug/ml.. IHC-P (10 - 15 µg/ml) Immunohistochemistry: LS-B3337 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3337 was determined to be 10 ug/ml.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID898
  • Target name
    CCNE1
  • Target description
    cyclin E1
  • Target synonyms
    CCNE, pCCNE1, G1/S-specific cyclin-E1
  • Host
    Rabbit
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-CCNE1 Antibody144-63138
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCCNE1
  • SizePrice
Figure 1. Western blot analysis of CCNE1 using anti-CCNE1 antibody (A00543-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PANC-1 whole cell lysates, Lane 7: human CACO-2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNE1 antigen affinity purified polyclonal antibody (Catalog # A00543-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNE1 at approximately 47KD. The expected band size for CCNE1 is at 47KD.
Product group Antibodies
Boster Bio
Anti-Cyclin E1/CCNE1 Antibody Picoband(r)A00543-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCNE1
  • SizePrice
Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin E1 antibody (GTX103045) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
Cyclin E1 antibodyGTX103045
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Monkey
TargetCCNE1
  • SizePrice
Product group Antibodies
Biomatik
CCNE1 Polyclonal AntibodyCAC14982
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetCCNE1
  • SizePrice
Product group Antibodies
Assay Genie
Phospho-Cyclin E1-T395 AntibodyCABP0013
ReactivityHuman
TargetCCNE1
  • SizePrice
HepG2 cells were stained with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Product group Antibodies
Bioss
Cyclin E1 Recombinant AntibodyBSM-52048R
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetCCNE1
  • SizePrice
Anti-Cyclin E1 Antibody
Product group Antibodies
Antibodies.com
Anti-Cyclin E1 AntibodyA94771
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
Cyclin E1 Antibody (YA483, Rabbit)HY-P80100
TargetCCNE1
  • SizePrice
Western Blot analysis of MCF7 cells using Cyclin E1 Polyclonal Antibody
Product group Antibodies
Cusabio
CCNE1 AntibodyCSB-PA001879
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCNE1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CCNE1 / Cyclin E1 AntibodyLS-C831032
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCCNE1
  • SizePrice