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ProductsBack/PathPlus(tm) ETFA Antibody (aa139-152)

PathPlus(tm) ETFA Antibody (aa139-152)

LS-B15950
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mammals, Monkey, Mouse, Rabbit, Rat, Xenopus, Zebra Fish
TargetETFA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) ETFA Antibody (aa139-152)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 30+35kD bands observed in Human Colon lysates and in Mouse and Rat Small Intestine lysates (calculated MW of 35.1kD according to NP_000117.1 and of 30.0kD according to NP_001121188.1. Recommended concentration: 0.1-0.3 ug/ml.. IHC-P (5 µg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 30+35kD bands observed in Human Colon lysates and in Mouse and Rat Small Intestine lysates (calculated MW of 35.1kD according to NP_000117.1 and of 30.0kD according to NP_001121188.1. Recommended concentration: 0.1-0.3 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2108
  • Target name
    ETFA
  • Target description
    electron transfer flavoprotein subunit alpha
  • Target synonyms
    EMA, GA2, MADD, electron transfer flavoprotein subunit alpha, mitochondrial, alpha-ETF, electron transfer flavoprotein alpha subunit, electron transfer flavoprotein, alpha polypeptide, epididymis secretory sperm binding protein, glutaric aciduria II, multiple acyl-CoA dehydrogenase deficiency
  • Host
    Goat
  • Reactivity
    Bovine, Canine, Equine, Human, Mammals, Monkey, Mouse, Rabbit, Rat, Xenopus, Zebra Fish
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ETFA using anti-ETFA antibody (A05572-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human U937 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETFA antigen affinity purified polyclonal antibody (Catalog # A05572-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETFA at approximately 35 kDa. The expected band size for ETFA is at 35 kDa.
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Western blot All lanes: ETFA antibody at 4.34microg/ml Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lane 4: A549 whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36, 31 kDa Observed band size: 36 kDa
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