PathPlus(tm) FOXP1 Antibody (clone JC12)

LifeSpan BioSciences

PathPlus(tm) FOXP1 Antibody (clone JC12)

14

Immunohistochemistry: LS-B2866 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2866 was determined to be 10 ug/ml. Histology Positive Control Tissue: Human tonsil. Western Blot: LS-B2866 detects a band of approximately 85kD in Tonsil extracts.. IHC-P (10 µg/ml), WB Immunohistochemistry: LS-B2866 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2866 was determined to be 10 ug/ml. Histology Positive Control Tissue: Human tonsil. Western Blot: LS-B2866 detects a band of approximately 85kD in Tonsil extracts.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

JC12

1 mg/ml

Unconjugated

FOXP1

forkhead box P1

12CC4, HSPC215, MFH, QRF1, hFKH1B, forkhead box protein P1, fork head-related protein like B, glutamine-rich factor 1, mac-1-regulated forkhead

Mouse

IgG2a

Human, Mouse

2°C to 8°C or -20°C

41116161