PathPlus(tm) MKI67 / Ki67 Antibody (aa1550-1700)

LifeSpan BioSciences

PathPlus(tm) MKI67 / Ki67 Antibody (aa1550-1700)

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This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. There have been mixed results in Western blot. *ormalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. This is probably the most critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear).. IF, IHC, IHC-Fr (1:50 - 1:300), IHC-P (5 µg/ml), WB This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. There have been mixed results in Western blot. *ormalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. This is probably the most critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear).

ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

MKI67

marker of proliferation Ki-67

KIA, MIB-, MIB-1, PPP1R105, proliferation marker protein Ki-67, Molecular Immunology Borstel antibody 1, antigen Ki67, antigen identified by monoclonal antibody Ki-67, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105

Rabbit

IgG

Human, Mouse, Porcine, Rat

-20°C,2°C to 8°C

41116161