More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PathPlus(tm) NF-L / NEFL Antibody (clone 2F11)

PathPlus(tm) NF-L / NEFL Antibody (clone 2F11)

LS-B7182
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Opossum
TargetNEFL
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) NF-L / NEFL Antibody (clone 2F11)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-Fr (1:25 - 1:200), IHC-P (10 µg/ml), WB (1:100 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2F11
  • Conjugate
    Unconjugated
  • Gene ID4747
  • Target name
    NEFL
  • Target description
    neurofilament light chain
  • Target synonyms
    CMT1F, CMT2E, CMTDIG, NF-L, NF68, NFL, PPP1R110, neurofilament light polypeptide, light molecular weight neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, neurofilament triplet L protein, neurofilament, light polypeptide 68kDa, protein phosphatase 1, regulatory subunit 110
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Opossum
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-Neurofilament light [MS-C2]AB00946-10.0-BT
ApplicationsELISA
ReactivityHuman
TargetNEFL
  • SizePrice
Product group Antibodies
RayBiotech
Anti-NEFL Antibody144-00257
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNEFL
  • SizePrice
Product group Antibodies
Biosynth
NEFL antibody10R-8366
  • SizePrice
Figure 1. Western blot analysis of NEFL/NF-L using anti-NEFL/NF-L antibody (A02482-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEFL/NF-L antigen affinity purified polyclonal antibody (Catalog # A02482-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEFL/NF-L at approximately 72KD. The expected band size for NEFL/NF-L is at 72KD.
Product group Antibodies
Boster Bio
Anti-NEFL/NF-L Antibody Picoband(r)A02482-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNEFL
  • SizePrice
Sandwich ELISA detection of recombinant full-length NF-L protein using GTX101142 as capture antibody at concentration of 5 μg/mL and GTX60544 as detection antibody was diluted at 1:10000. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
Product group Antibodies
GeneTex
NF-L antibodyGTX101142
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetNEFL
  • SizePrice
Product group Antibodies
Biomatik
Nefl Polyclonal AntibodyCAC08666
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
TargetNEFL
  • SizePrice
Product group Antibodies
Assay Genie
Neurofilament L AntibodyCAB0257
ReactivityHuman
TargetNEFL
  • SizePrice
Product group Antibodies
Bioss
NF-L Polyclonal AntibodyBS-0707R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetNEFL
  • SizePrice
Anti-NEFL Antibody
Product group Antibodies
Antibodies.com
Anti-NEFL AntibodyA28063
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
  • SizePrice
Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using Anti-Neurofilament-L (NF-L) Rabbit Monoclonal Antibody (Clone RM280) at a 1:2500 dilution.
Product group Antibodies
RevMAb Biosciences
anti-Neurofilament-L (human), Rabbit Monoclonal (RM280)REV-31-1163-00
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetNEFL
  • SizePrice