PathPlus(tm) OCLN / Occludin Antibody (C-Terminus)

LifeSpan BioSciences

PathPlus(tm) OCLN / Occludin Antibody (C-Terminus)

23

Immunohistochemistry: LS-B2187 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2187 was determined to be 2.5 ug/ml.. ELISA (0.1 - 1 µg/ml), IF, IHC-P (2.5 µg/ml), IP, WB (1 - 3 µg/ml) Immunohistochemistry: LS-B2187 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2187 was determined to be 2.5 ug/ml.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.25 mg/ml

Unconjugated

OCLN

occludin

BLCPMG, PPP1R115, PTORCH1, occludin, phosphatase 1, regulatory subunit 115

Rabbit

IgG

Canine, Human, Mouse, Rat

-20°C,2°C to 8°C

41116161