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ProductsBack/PathPlus(tm) THBD / CD141 / Thrombomodulin Antibody (C-Terminus)

PathPlus(tm) THBD / CD141 / Thrombomodulin Antibody (C-Terminus)

LS-B12463
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTHBD
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) THBD / CD141 / Thrombomodulin Antibody (C-Terminus)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:100 - 1:1000), IHC, IHC-P (10 - 20 µg/ml), WB (1:500 - 1:3000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7056
  • Target name
    THBD
  • Target description
    thrombomodulin
  • Target synonyms
    AHUS6, BDCA-3, BDCA3, CD141, THPH12, THRM, TM, thrombomodulin, CD141 antigen, fetomodulin
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (A01325-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombomodulin/THBD antigen affinity purified polyclonal antibody (Catalog # A01325-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombomodulin/THBD at approximately 100 kDa. The expected band size for Thrombomodulin/THBD is at 60 kDa.
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