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THBD Antibody

CAB4155
Assay Genie
ReactivityHuman
Product group Antibodies
TargetTHBD
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Overview

  • Supplier
    Assay Genie
  • Product Name
    THBD Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID7056
  • Target name
    THBD
  • Target description
    thrombomodulin
  • Target synonyms
    AHUS6, BDCA-3, BDCA3, CD141, THPH12, THRM, TM, thrombomodulin, CD141 antigen, fetomodulin
  • Host
    Rabbit
  • Protein IDP07204
  • Protein Name
    Thrombomodulin
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: THBD antibody at 3.07microg/ml Lane 1: Rat heart tissue Lane 2: Mouse kidney tissue Lane 3: A549 whole cell lysate Lane 4: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa
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Figure 1. Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (A01325-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombomodulin/THBD antigen affinity purified polyclonal antibody (Catalog # A01325-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombomodulin/THBD at approximately 100 kDa. The expected band size for Thrombomodulin/THBD is at 60 kDa.
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Lane 1: Lung lysates; Lane 2: Placenta lysates probed with Thrombomodulin Polyclonal Antibody, Unconjugated (bs-20395R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
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