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ProductsBack/PAX-8 (Paired box protein 8)

PAX-8 (Paired box protein 8)

PAX008-0.5
Nordic-MUbio
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPAX8
0.5 ml
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    PAX-8 (Paired box protein 8)
  • Delivery Days Customer
    7
  • Application Supplier Note
    IHC (C,P) Staining procedures: Use formalin-fixed and paraffin-embedded sections. Retrieval conditions: Pretreatment of deparaffinized tissue with heat-induced epitope retrieval (EDTA, pH9.0) is recommended. Detection methods: Polymer anti-mouse/rabbit Ig detection system. Working dilution: 1:50-100; Positive Control: Ovarian serous carcinoma. Cellular Localization: Nuclear.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunohistochemistry (frozen);Immunohistochemistry (paraffin)
  • Category Supplier
    Primary Antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ZR1
  • Gene ID7849
  • Target name
    PAX8
  • Target description
    paired box 8
  • Target synonyms
    paired box protein Pax-8; paired domain gene 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06710
  • Protein Name
    Paired box protein Pax-8
  • Scientific Description
    Paired box protein 8
  • Source
    Rabbits were immunized with a synthetic peptide corresponding to the C-terminus of Human PAX8 protein.
  • Reactivity Supplier
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAX8 using anti-PAX8 antibody (A00943-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW579 cell lysate, Lane 2: human SW579 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX8 antigen affinity purified polyclonal antibody (Catalog # A00943-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX8 at approximately 48KD. The expected band size for PAX8 is at 48KD.
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