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ProductsBack/PBP (PEBP1) Mouse Monoclonal Antibody [Clone ID: OTI2D4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PEBP1 (Cat# RC206355, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEBP1(Cat# TA501616). Positive lysates [LY400913] (100ug) and [LC400913] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PEBP1 (Cat# RC206355, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEBP1(Cat# TA501616). Positive lysates [LY400913] (100ug) and [LC400913] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PEBP1 (Cat# RC206355, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEBP1(Cat# TA501616). Positive lysates [LY400913] (100ug) and [LC400913] (20ug) can be purchased separately from OriGene.

PBP (PEBP1) Mouse Monoclonal Antibody [Clone ID: OTI2D4]

CF501616
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetPEBP1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    PBP (PEBP1) Mouse Monoclonal Antibody [Clone ID: OTI2D4]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2D4
  • Gene ID5037
  • Target name
    PEBP1
  • Target description
    phosphatidylethanolamine binding protein 1
  • Target synonyms
    HCNP, HCNPpp, HEL-210, HEL-S-34, HEL-S-96, PBP, PEBP, PEBP-1, RKIP, phosphatidylethanolamine-binding protein 1, Raf kinase inhibitory protein, epididymis luminal protein 210, epididymis secretory protein Li 34, epididymis secretory protein Li 96, hippocampal cholinergic neurostimulating peptide, neuropolypeptide h3, prostatic binding protein, raf kinase inhibitor protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP30086
  • Protein Name
    Phosphatidylethanolamine-binding protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PEBP1 mouse monoclonal antibody, clone OTI2D4 (formerly 2D4)
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PBP using anti-PBP antibody (A01668). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: mouse testis lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBP antigen affinity purified polyclonal antibody (Catalog # A01668) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PBP at approximately 21 kDa. The expected band size for PBP is at 21 kDa.
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