ICC/IF analysis of GM0637 cells using GTX00723 PCNA antibody. Green : Primary antibody Blue : Hoechst 33342 Dilution : 1:100 Fixation : 4% PFA Permeabilization : 0.5% Triton X-100
ICC/IF analysis of GM0637 cells using GTX00723 PCNA antibody. Green : Primary antibody Blue : Hoechst 33342 Dilution : 1:100 Fixation : 4% PFA Permeabilization : 0.5% Triton X-100
PCNA antibody
GTX00723
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityChicken, Hamster, Human, Mouse, Rat, Xenopus
TargetPCNA
Overview
- SupplierGeneTex
- Product NamePCNA antibody
- Delivery Days Customer9
- Antibody SpecificityThis antibody could detect 0.3 ng of PCNA by dot blot.
- Application Supplier NoteWB: 1:1000-1:2000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Dot: 1:500-1:2000. ELISA: 1:500-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5111
- Target namePCNA
- Target descriptionproliferating cell nuclear antigen
- Target synonymsATLD2; cyclin; DNA polymerase delta auxiliary protein; proliferating cell nuclear antigen
- HostRabbit
- IsotypeIgG
- Protein IDP12004
- Protein NameProliferating cell nuclear antigen
- Scientific DescriptionThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
- ReactivityChicken, Hamster, Human, Mouse, Rat, Xenopus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203